Plasmid

Part:BBa_I713029:Design

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-26)


pEB-Mob


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1378
    Illegal SpeI site found at 258
    Illegal PstI site found at 1338
    Illegal PstI site found at 2528
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 258
    Illegal PstI site found at 1338
    Illegal PstI site found at 2528
    Illegal NotI site found at 840
    Illegal NotI site found at 1359
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2315
    Illegal BamHI site found at 252
    Illegal BamHI site found at 299
    Illegal XhoI site found at 1366
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1378
    Illegal SpeI site found at 258
    Illegal PstI site found at 1338
    Illegal PstI site found at 2528
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1378
    Illegal SpeI site found at 258
    Illegal PstI site found at 1338
    Illegal PstI site found at 2528
    Illegal NgoMIV site found at 1704
    Illegal NgoMIV site found at 2979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 520
    Illegal BsaI site found at 3878
    Illegal SapI.rc site found at 2828
    Illegal SapI.rc site found at 3038


Design Notes

NcoI - Mob Gene - BamHI


Source

pBBR1MCS

References